Journal: Brain : a journal of neurology

Article Title: Connexin43 mimetic peptide reduces vascular leak and retinal ganglion cell death following retinal ischaemia.

doi: 10.1093/brain/awr338

Figure Lengend Snippet: Figure 3 Three hours of hypoxia and 6 h of reperfusion lead to significant endothelial cell death in vitro in rat brain microvascular endothelial cells (RBMVEC; A). Non-specific gap junction blocker carbenoxolone, non-specific hemichannel blocker LaCl3, and connexin43 (Cx43) mimetic peptide protected endothelial cells against hypoxic injury, with the number of viable cells significantly higher than no treatment, while scrambled peptide did not have any protective effects. The number of viable cells was expressed as percentage of the control without hypoxia. Hypoxia and reperfusion also lead to significant propidium iodide dye uptake into primary rat brain microvascular endothelial cells indicating open hemichannels (B). Carbenoxolone, LaCl3 and connexin43 mimetic peptide significantly prevented dye uptake compared to no treatment, indicating hemichannel closure, while scrambled peptide did not have any effect. Stars denote statistical significance when compared to the control group or compared between groups in brackets; P 5 0.05.

Article Snippet: Rat brain microvascular endothelial cells (R840K-05a, Cell Applications) were plated into 24-well plates (1 105 cells/well) in rat brain microvascular endothelial cell growth media (R819K-500, Cell Applications) and allowed to settle for 16 h. Medium was then removed and replaced with Dulbecco’s Modified Eagle’s Medium/F12 containing 0.5% foetal bovine serum and 1% glutamine.

Techniques: In Vitro, Control

Journal: BMC Cancer

Article Title: LncRNA MT1JP plays a protective role in intrahepatic cholangiocarcinoma by regulating miR-18a-5p/FBP1 axis

doi: 10.1186/s12885-021-07838-0

Figure Lengend Snippet: MT1JP inhibited proliferation and promoted apoptosis in intrahepatic cholangiocarcinoma cells. A and B. The levels of MT1JP and miR-18a-5p were detected by real-time PCR in normal primary intrahepatic cholangetic epithelial cells and several intrahepatic cholangiocarcinoma cell lines. C. The MT1JP levels were verified in HCCC-9810 and HUCCT1 cells after overexpression or knockdown. D and E. CCK-8 assay was used for detection of cell viability. F. The PCNA expression levels were detected by western blot after ectopic expression or silencing of MT1JP. (The western blot bands were cropped from Fig. and .) G. Flow cytometry was used for cell distribution in each phase. H. The expression levels of several cell cycle proteins were detected. (The western blot bands were cropped from Fig. , , and .) I. Flow cytometry was performed for detection of cell apoptosis. J. The expression levels of several apoptosis proteins were examined after MT1JP overexpression in HCCC-9810 cells. (The western blot bands were cropped from Fig. and .) ( *p < 0.05, **p < 0.01, ***p < 0.001 , compared with normal, pcDNA3.1 or siRNA NC; the original images of western blot were shown in supplementary Fig. 1–8)

Article Snippet: Primary intrahepatic cholangetic epithelial cells were purchased from iCell (Shanghai, China), and cultured with primary epithelial cell medium (iCell) at 37 °C with 5% CO 2 .

Techniques: Real-time Polymerase Chain Reaction, Over Expression, Knockdown, CCK-8 Assay, Expressing, Western Blot, Flow Cytometry

Journal: bioRxiv

Article Title: A mechanism of melanogenesis mediated by E-cadherin downregulation and its involvement in solar lentigines

doi: 10.1101/2023.01.09.523359

Figure Lengend Snippet: A, B , Cultured keratinocytes, but not cultured melanocytes, significantly promoted proliferation in the inhibition of E-cadherin by a neutralizing antibody against E-cadherin (α-E-cad.). C , In the melanocyte monoculture, there was no effect on melanogenesis in E-cadherin neutralization. D , In the keratinocyte-melanocyte coculture, melanocytes promoted melanogenesis with upregulation of Trp1 protein expression, suggesting that the promotion of melanogenesis is dependent on the presence of E-cadherin-inhibited keratinocytes. BF; Bright field. Scale Bars, 100 µm. The data represents mean±SD.

Article Snippet: The siRNA-treated keratinocytes were further incubated by replacing the medium with the coculture medium (CnT-PRIME KM, CELLnTEC) for the coculture with melanocytes.

Techniques: Cell Culture, Inhibition, Neutralization, Expressing

Journal: bioRxiv

Article Title: A mechanism of melanogenesis mediated by E-cadherin downregulation and its involvement in solar lentigines

doi: 10.1101/2023.01.09.523359

Figure Lengend Snippet: A - C , By coculture with either control or E-cadherin knockdown keratinocytes (E-cad-KD), melanocytes cocultured with E-cad-KD keratinocytes promoted melanogenesis more than ones cocultured with control keratinocytes. D - F , Melanocytes cocultured with both control and E-cad-KD keratinocytes showed no obvious morphological changes in the total number of dendrite per cell, the length of individual dendrites, and the area per cell. Scale Bars, 100 µm. The data represents mean±SD.

Article Snippet: The siRNA-treated keratinocytes were further incubated by replacing the medium with the coculture medium (CnT-PRIME KM, CELLnTEC) for the coculture with melanocytes.

Techniques: Control, Knockdown